Western blot analysis of extracts of HepG2 cells, using SF1 antibody (A6424) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SF1 Rabbit pAb
Catalog No:AB-A6424
Manufacturer:ABclonal
Synonyms:SF1;BBP;D11S636;MBBP;ZCCHC25;ZFM1;ZNF162
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1). |
Gene ID: | 7536 |
Swiss prot: | Q15637 |
Molecular weight: | 59kDa/61kDa/65kDa/67kDa/68kDa/71kDa |
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