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Western blot analysis of extracts of various cell lines, using Septin 5 antibody (A12953) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using Septin 5 antibody (A12953) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Septin 5 antibody (A12953) at dilution of 1:100 (40x lens).
Septin 5 Rabbit pAb
Catalog No：AB-A12953
Manufacturer：ABclonal
Synonyms：SEPT5;CDCREL;CDCREL-1;CDCREL1;H5;HCDCREL-1;PNUTL1;septin-5
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Septin 5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human Septin 5 (NP_002679.2). |
| Gene ID: | 5413 |
| Swiss prot: | Q99719 |
| Molecular weight: | 39kDa/42kDa |
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