Western blot analysis of extracts of various cell lines, using SEMA4G antibody (A15492) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
SEMA4G Rabbit pAb
Catalog No:AB-A15492
Manufacturer:ABclonal
Synonyms:SEMA4G
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SEMA4G Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-560 of human SEMA4G (NP_001190173.1). |
Gene ID: | 57715 |
Swiss prot: | Q9NTN9 |
Molecular weight: | 77kDa/91kDa/92kDa |
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