Western blot analysis of extracts of various cell lines, using SELENOK antibody (A20560) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
SELENOK Rabbit pAb
Catalog No:AB-A20560
Manufacturer:ABclonal
Synonyms:SELK; HSPC030; HSPC297
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€125,00 – €370,00
Product Name: | SELENOK Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the selenoprotein K family. It is a transmembrane protein that is localized in the endoplasmic reticulum (ER), and is involved in ER-associated degradation (ERAD) of misfolded, glycosylated proteins. It also has a role in the protection of cells from ER stress-induced apoptosis. Knockout studies in mice show the importance of this gene in promoting Ca(2+) flux in immune cells and mounting effective immune response. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3′ UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Pseudogenes of this locus have been identified on chromosomes 6 and 19. |
Immunogen: | Recombinant protein of human SELENOK. |
Gene ID: | 58515 |
Swiss prot: | Q9Y6D0 |
Molecular weight: | 10kDa |
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