Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD mAb (A20141) with a linear range of 0.78-50ng/mL.

Western blot analysis of extracts of normal 293T cells 293T transfected with Spike RBD Protein, using SARS-COV-2 Spike RBD antibody (A20141) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.

Immunofluorescence analysis of 293T cells transfected with SARS-COV-2 Spike RBD protein and untreated 293T cells use SARS-COV-2 Spike RBD Mouse mAb (A20141) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.