Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike ECD Protein,using SARS-CoV-2 Spike antibody (A20136) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.

Immobilized Recombinant SARS-COV-2 S1+S2 ECD(S-ECD) Protein (RP01283) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike Rabbit pAb (A20137) with a linear range of 0.78-50ng/mL.

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike antibody (A20137). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (A20137) at a dilition of 1:1000.

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike protein and untreated 293T cells use SARS-CoV-2 Spike Rabbit pAb (A20137) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.