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Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.
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Western blot analysis of extracts of normal 293T cells 293T transfected with N Protein, using SARS-COV-2 N Protein antibody (A20142) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
SARS-CoV-2 N Protein Mouse mAb
Catalog No：AB-A20142
Manufacturer：ABclonal
Synonyms：Nucleoprotein, NP
Applications：ELISA,WB
Reactivity：SARS-CoV-2
Host：Mouse
Conjugate：No
€65,00 – €370,00
| Product Name: | SARS-CoV-2 N Protein Mouse mAb |
| Category: | Monoclonal Antibodies |
| Description: | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Immunogen: | Recombinant fusion protein of SARS-CoV-2 Nucleoprotein. |
| Gene ID: | 1489678 |
| Swiss prot: | P59595 |
| Molecular weight: |
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