Western blot analysis of extracts of various cell lines, using RPN1 antibody (A12497) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RPN1 Rabbit pAb
Catalog No:AB-A12497
Manufacturer:ABclonal
Synonyms:RPN1;OST1;RBPH1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RPN1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1). |
Gene ID: | 6184 |
Swiss prot: | P04843 |
Molecular weight: | 68kDa |
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