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Western blot analysis of extracts of various cell lines, using RPL5 antibody (A1977) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody (A1977) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody (A1977) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RPL5 antibody (A1977). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody (A1977) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody (A1977) at dilution of 1:200 (40x lens).
RPL5 Rabbit pAb
Catalog No：AB-A1977
Manufacturer：ABclonal
Synonyms：RPL5;DBA6;L5;MSTP030;PPP1R135
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | RPL5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2). |
| Gene ID: | 6125 |
| Swiss prot: | P46777 |
| Molecular weight: | 34kDa |
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