Western blot analysis of extracts of various cell lines, using RPL28 antibody (A15095) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
RPL28 Rabbit pAb
Catalog No:AB-A15095
Manufacturer:ABclonal
Synonyms:RPL28;L28
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RPL28 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPL28 (NP_000982.2). |
Gene ID: | 6158 |
Swiss prot: | P46779 |
Molecular weight: | 7kDa/10kDa/15kDa/18kDa/19kDa |
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