Western blot analysis of extracts of various cell lines, using RPL14 antibody (A6724) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
RPL14 Rabbit pAb
Catalog No:AB-A6724
Manufacturer:ABclonal
Synonyms:RPL14;CAG-ISL-7;CTG-B33;L14;RL14;hRL14
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RPL14 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5′-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3). |
Gene ID: | 9045 |
Swiss prot: | P50914 |
Molecular weight: | 23kDa |
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