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Western blot analysis of extracts of various cell lines, using RPA70 antibody (A9841) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
RPA70 Rabbit pAb
Catalog No：AB-A9841
Manufacturer：ABclonal
Synonyms：RPE65;BCO3;LCA2;RP20;mRPE65;rd12;sRPE65
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | RPA70 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 274-533 of human RPA70 (NP_000320.1). |
| Gene ID: | 6121 |
| Swiss prot: | Q16518 |
| Molecular weight: | 60kDa |
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