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Western blot analysis of extracts of various cell lines, using ROCK1 Rabbit mAb (A11158) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
ROCK1 Rabbit mAb
Catalog No：AB-A11158
Manufacturer：ABclonal
Synonyms：ROCK1;P160ROCK;ROCK-I
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €540,00
| Product Name: | ROCK1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. |
| Immunogen: | Recombinant protein of human ROCK1 |
| Gene ID: | 6093 |
| Swiss prot: | Q13464 |
| Molecular weight: | 158kDa |
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