Western blot analysis of extracts of various cell lines, using RNF20 antibody (A17309) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RNF20 Rabbit pAb
Catalog No:AB-A17309
Manufacturer:ABclonal
Synonyms:RNF20;BRE1;BRE1A;hBRE1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RNF20 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF20 (NP_062538.5). |
Gene ID: | 56254 |
Swiss prot: | Q5VTR2 |
Molecular weight: | 113kDa |
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