Western blot analysis of extracts of various cell lines, using RhoGAP antibody (A3587) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
RhoGAP Rabbit pAb
Catalog No:AB-A3587
Manufacturer:ABclonal
Synonyms:ARHGAP5;GFI2;RhoGAP5;p190-B;p190BRhoGAP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RhoGAP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human RhoGAP (NP_001164.2). |
Gene ID: | 394 |
Swiss prot: | Q13017 |
Molecular weight: | 26kDa/27kDa/172kDa |
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