Western blot analysis of extracts of various cell lines, using RhoA antibody (A0272) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RhoA Rabbit pAb
Catalog No:AB-A0272
Manufacturer:ABclonal
Synonyms:ARH12;ARHA;RHO12;RHOH12;Rho;RHOA;RhoA
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RhoA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RhoA (NP_001655.1). |
Gene ID: | 387 |
Swiss prot: | P61586 |
Molecular weight: | 21kDa |
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