Western blot analysis of extracts of various cell lines, using RFC2 antibody (A6847) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
RFC2 Rabbit pAb
Catalog No:AB-A6847
Manufacturer:ABclonal
Synonyms:RFC2;RFC40
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RFC2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 38-297 of human RFC2 (NP_852136.1). |
Gene ID: | 5982 |
Swiss prot: | P35250 |
Molecular weight: | 35kDa/39kDa |
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