Western blot analysis of extracts of HeLa cells, using RFC1 antibody (A1625) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using RFC1 antibody (A1625) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
RFC1 Rabbit pAb
Catalog No:AB-A1625
Manufacturer:ABclonal
Synonyms:RFC1;A1;MHCBFB;PO-GA;RECC1;RFC;RFC140
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RFC1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3′ end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1). |
Gene ID: | 5981 |
Swiss prot: | P35251 |
Molecular weight: | 128kDa |
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