Renin FRET Substrate I
Catalog No：CP-RENI-013
Manufacturer：CPC Scientific
Size：1 mg
MWt：1,798
€235,00
| Product Name: | Renin FRET Substrate I |
| Category: | Peptides |
| Three letter sequence: | DABCYL- gama-Abu- Ile-His-Pro- Phe-His-Leu- Val-Ile-His- Thr-EDANS |
| One letter sequence: | DABCYL-g-Abu- IHPFHLVIHT- EDANS |
| Description: | DABCYL-GABA-Ile- His-Pro-Phe-His- Leu-Val-Ile-His-Thr- EDANS (also called Renin Substrate I in some literature) contains a renin cleavage site that occurs in the N- terminal peptide of human angiotensinogen. Cleavage of the substrate occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen. This fluorogenic peptide substrate is used to continuously measure the proteolytic activity of human renin. The assay relies upon FRET-mediated, |
| Literature References: | Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9, Nakamura N, et al. (1991). Identification of the active site of human renin with use of new fluorogenic peptides. J Biochem (Tokyo) 109, 741-5, Murakami K, et al. (1981). New fluorogenic substrates for renin. Anal Biochem 110, 232-9. |
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