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Recombinant Human TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 17 kDa.
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Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/mL.
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The purity of Human TNF-alpha Protein (Cat.RP00001) was greater than 90% as determined by SEC-HPLC.
Recombinant Human TNF-alpha Protein
Catalog No：AB-RP00001
Manufacturer：ABclonal
Synonyms：DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F
Expression Host：E. coli
€38,00 – €340,00
| Product Name: | Recombinant Human TNF-alpha Protein |
| Category: | Proteins/Peptides > TNF family,Cell Culture related,Drug Targets |
| Description: | Recombinant Human TNF-alpha Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val77-Leu233) of human TNF-alpha (Accession #NP_000585.2) fused with an initial Met at the N-terminus and a 6xHis tag at the C-terminus. |
| Gene ID: | 67124 |
| Swiss prot: | P01375 |
| Bioactivity: | Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/mL. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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