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Recombinant Human IGF1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 41 kDa.
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Recombinant Human IGF1 promotes the proliferation of MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL.
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Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1ug/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250ng/mL.
Recombinant Human IGF1 Protein
Catalog No：AB-RP00996
Manufacturer：ABclonal
Synonyms：IGF-1;IGF-I;IGF1A;IGFI;IGFI;MGF
Expression Host：HEK293 cells
€26,00 – €170,00
| Product Name: | Recombinant Human IGF1 Protein |
| Category: | Proteins/Peptides > Growth factor,Cell Culture related,Drug Targets |
| Description: | Recombinant Human IGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49 – Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6xHis tag at the C-terminus. |
| Gene ID: | 4763479 |
| Swiss prot: | P05019-1 |
| Bioactivity: | Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells.0.01-1ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.|Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1ug/mL (100 μL/well) can bind recombinant human IGF1 with a linear range of 30-250ng/mL.|Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 7.5-30 ng/mL. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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