Western blot analysis of extracts of 293T cells, using Raptor antibody (A16309) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5min.
Raptor Rabbit pAb
Catalog No:AB-A16309
Manufacturer:ABclonal
Synonyms:KOG1;Mip1;RPTOR
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Raptor Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant protein of human RPTOR |
Gene ID: | 57521 |
Swiss prot: | Q8N122 |
Molecular weight: | 43kDa/131kDa/149kDa |
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