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Western blot analysis of extracts of Mouse liver, using Raf1 antibody (A0223) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Raf1 Rabbit pAb (A0223) at dilution of 1:100 (40x lens).
Raf1 Rabbit pAb
Catalog No：AB-A0223
Manufacturer：ABclonal
Synonyms：CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Raf1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human Raf1 (NP_002871.1). |
| Gene ID: | 5894 |
| Swiss prot: | P04049 |
| Molecular weight: | 73kDa/75kDa |
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