Western blot analysis of extracts of various cell lines, using Rad52 antibody (A3077) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Rad52 Rabbit pAb
Catalog No:AB-A3077
Manufacturer:ABclonal
Synonyms:RAD52
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Rad52 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human Rad52 (NP_602296.2). |
Gene ID: | 5893 |
Swiss prot: | P43351 |
Molecular weight: | 12kDa/15kDa/24kDa/46kDa |
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