Western blot analysis of extracts of various cell lines, using RAD51B antibody (A6962) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
RAD51B Rabbit pAb
Catalog No:AB-A6962
Manufacturer:ABclonal
Synonyms:RAD51B;R51H2;RAD51L1;REC2
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RAD51B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-384 of human RAD51B (NP_598193.2). |
Gene ID: | 5890 |
Swiss prot: | O15315 |
Molecular weight: | 25kDa/38kDa/40kDa/42kDa |
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