Western blot analysis of extracts of various cell lines, using Rad50 Rabbit mAb (A3869) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Rad50 Rabbit mAb
Catalog No:AB-A3869
Manufacturer:ABclonal
Synonyms:NBSLD, RAD502, hRad50
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Rad50 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010] |
Immunogen: | A synthesized peptide derived from human Rad50 |
Gene ID: | 10111 |
Swiss prot: | Q92878 |
Molecular weight: | 154kDa |
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