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Western blot analysis of extracts of various cell lines, using Rad21 Rabbit mAb (A19749) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Rad21 Rabbit mAb
Catalog No：AB-A19749
Manufacturer：ABclonal
Synonyms：CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | Rad21 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Rad21 |
| Gene ID: | 5885 |
| Swiss prot: | O60216 |
| Molecular weight: | kDa |
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