Western blot analysis of extracts of various cell lines, using Rad18 Rabbit mAb (A4339) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Rad18 Rabbit mAb
Catalog No:AB-A4339
Manufacturer:ABclonal
Synonyms:RNF73
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Rad18 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human Rad18 |
Gene ID: | 56852 |
Swiss prot: | Q9NS91 |
Molecular weight: | 56kDa |
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