Western blot analysis of extracts of various cell lines, using PUS10 antibody (A15962) at 1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PUS10 Rabbit pAb
Catalog No:AB-A15962
Manufacturer:ABclonal
Synonyms:PUS10;CCDC139;DOBI
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PUS10 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2). |
Gene ID: | 150962 |
Swiss prot: | Q3MIT2 |
Molecular weight: | 60kDa |
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