Western blot analysis of extracts of various cell lines, using PUF60 antibody (A6709) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PUF60 Rabbit pAb
Catalog No:AB-A6709
Manufacturer:ABclonal
Synonyms:PUF60;FIR;RoBPI;SIAHBP1;VRJS
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PUF60 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2). |
Gene ID: | 22827 |
Swiss prot: | Q9UHX1 |
Molecular weight: | 54kDa/55kDa/57kDa/58kDa/59kDa |
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