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Western blot analysis of extracts of various cell lines, using PTK2B antibody (A16045) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PTK2B Rabbit pAb
Catalog No：AB-A16045
Manufacturer：ABclonal
Synonyms：PTK2B;CADTK;CAKB;FADK2;FAK2;PKB;PTK;PYK2;RAFTK;Pyk2
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PTK2B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTK2B (NP_004094.3). |
| Gene ID: | 2185 |
| Swiss prot: | Q14289 |
| Molecular weight: | 111kDa/115kDa |
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