Western blot analysis of extracts of various cell lines, using PSMC6 antibody (A5377) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PSMC6 Rabbit pAb
Catalog No:AB-A5377
Manufacturer:ABclonal
Synonyms:PSMC6;CADP44;HEL-S-73;P44;SUG2;p42;ATPase 6
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PSMC6 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PSMC6 (NP_002797.3). |
Gene ID: | 5706 |
Swiss prot: | P62333 |
Molecular weight: | 44kDa |
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