Western blot analysis of extracts of various cell lines, using PRPF8 antibody (A6053) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
PRPF8 Rabbit pAb
Catalog No:AB-A6053
Manufacturer:ABclonal
Synonyms:PRPF8;HPRP8;PRP8;PRPC8;RP13;SNRNP220;PRPF8
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRPF8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). |
Gene ID: | 10594 |
Swiss prot: | Q6P2Q9 |
Molecular weight: | 273kDa |
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