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Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) Rabbit pAb (A13447) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Prolyl hydroxylase PHD1 (EGLN2) Rabbit pAb
Catalog No：AB-A13447
Manufacturer：ABclonal
Synonyms：EGLN2;EIT6;HIF-PH1;HIFPH1;HPH-1;HPH-3;PHD1
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Prolyl hydroxylase PHD1 (EGLN2) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) (NP_444274.1). |
| Gene ID: | 112398 |
| Swiss prot: | Q96KS0 |
| Molecular weight: | 40kDa/43kDa |
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Conjugate：No
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Reactivity：Human,Mouse,Rat
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Conjugate：No
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Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
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