Western blot analysis of extracts of various cell lines, using PRDM9 antibody (A20340) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PRDM9 Rabbit pAb
Catalog No:AB-A20340
Manufacturer:ABclonal
Synonyms:PFM6; KMT8B; MSBP3; ZNF899; MEISETZ
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€125,00 – €370,00
Product Name: | PRDM9 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. |
Immunogen: | Recombinant protein of human PRDM9. |
Gene ID: | 56979 |
Swiss prot: | Q9NQV7 |
Molecular weight: | 103kDa |
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