Western blot analysis of extracts of various cell lines, using PRDM14 antibody (A5543) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Western blot analysis of extracts of NCI-H125 cells, using PRDM14 antibody (A5543) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PRDM14 Rabbit pAb
Catalog No:AB-A5543
Manufacturer:ABclonal
Synonyms:PRDM14;PFM11
Applications:WB,IHC,IF,IP,ChIP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRDM14 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-571 of human PRDM14 (AAH52311.1). |
Gene ID: | 63978 |
Swiss prot: | Q9GZV8 |
Molecular weight: | 64kDa |
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