Western blot analysis of extracts of various cell lines, using PPL antibody (A6950) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
PPL Rabbit pAb
Catalog No:AB-A6950
Manufacturer:ABclonal
Synonyms:PPL
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PPL Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3). |
Gene ID: | 5493 |
Swiss prot: | O60437 |
Molecular weight: | 204kDa |
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