Western blot analysis of extracts of various cell lines, using PPARγ antibody (A0270) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Rat fat, using PPARγ antibody (A0270) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PPARγ Rabbit pAb
Catalog No:AB-A0270
Manufacturer:ABclonal
Synonyms:CIMT1;GLM1;NR1C3;PPARG1;PPARG2;PPARgamma;PPAR gamma;PPARG
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PPARγ Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPARγ (NP_056953.2). |
Gene ID: | 5468 |
Swiss prot: | P37231 |
Molecular weight: | 21kDa/54kDa/57kDa |
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