Western blot analysis of extracts of various cell lines, using PORCN antibody (A12120) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
PORCN Rabbit pAb
Catalog No:AB-A12120
Manufacturer:ABclonal
Synonyms:PORCN;DHOF;FODH;MG61;PORC;PPN
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PORCN Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1). |
Gene ID: | 64840 |
Swiss prot: | Q9H237 |
Molecular weight: | 42kDa/51kDa/52kDa |
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