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Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
POLR2B Rabbit pAb
Catalog No：AB-A5928
Manufacturer：ABclonal
Synonyms：POLR2B;POL2RB;RPB2;hRPB140
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | POLR2B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1). |
| Gene ID: | 5431 |
| Swiss prot: | P30876 |
| Molecular weight: | 133kDa |
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