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Western blot analysis of extracts of various cell lines, using POLR2A antibody (A11181) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (A11181) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (A11181) at dilution of 1:100 (40x lens).
POLR2A Rabbit pAb
Catalog No：AB-A11181
Manufacturer：ABclonal
Synonyms：POLR2A;POLR2;POLRA;RPB1;RPBh1;RPO2;RPOL2;RpIILS;hRPB220;hsRPB1;Pol II
Applications：WB,IHC,IF,IP,ChIP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | POLR2A Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1). |
| Gene ID: | 5430 |
| Swiss prot: | P24928 |
| Molecular weight: | 63kDa/217kDa |
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