Western blot analysis of extracts of various cell lines, using POLR1B antibody (A12034) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
POLR1B Rabbit pAb
Catalog No:AB-A12034
Manufacturer:ABclonal
Synonyms:POLR1B;RPA135;RPA2;Rpo1-2
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLR1B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). |
Gene ID: | 84172 |
Swiss prot: | Q9H9Y6 |
Molecular weight: | 104kDa/107kDa/121kDa/128kDa/132kDa |
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