Western blot analysis of extracts of various cell lines, using POLD1 antibody (A5323) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
POLD1 Rabbit pAb
Catalog No:AB-A5323
Manufacturer:ABclonal
Synonyms:POLD1;CDC2;CRCS10;MDPL;POLD
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | POLD1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3′ to 5′ exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2). |
Gene ID: | 5424 |
Swiss prot: | P28340 |
Molecular weight: | 123kDa |
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