Western blot analysis of extracts of various cell lines, using PLOD1 antibody (A7919) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
PLOD1 Rabbit pAb
Catalog No:AB-A7919
Manufacturer:ABclonal
Synonyms:PLOD1;EDS6;LH;LH1;LLH;PLOD
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PLOD1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_000293.2). |
Gene ID: | 5351 |
Swiss prot: | Q02809 |
Molecular weight: | 83kDa/88kDa |
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