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Western blot analysis of extracts of various cell lines, using PLC gamma 1 (PLC gamma 1 (PLCG1)) Rabbit pAb (A16953) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PLC gamma 1 (PLCG1) Rabbit pAb
Catalog No：AB-A16953
Manufacturer：ABclonal
Synonyms：PLCG1;NCKAP3;PLC-II;PLC1;PLC148;PLCgamma1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PLC gamma 1 (PLCG1) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide of human PLC gamma 1 (PLC gamma 1 (PLCG1)). |
| Gene ID: | 5335 |
| Swiss prot: | P19174 |
| Molecular weight: | 148kDa |
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