Western blot analysis of extracts of HeLa cells, using Placental alkaline phosphatase (PLAP) antibody (A6353) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Placental alkaline phosphatase (PLAP) Rabbit pAb
Catalog No:AB-A6353
Manufacturer:ABclonal
Synonyms:ALPP;ALP;PALP;PLAP;PLAP-1;placental
Applications:WB,IHC,IP
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Placental alkaline phosphatase (PLAP) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human Placental alkaline phosphatase (PLAP) (NP_001623.3). |
Gene ID: | 250 |
Swiss prot: | P05187 |
Molecular weight: | 57kDa |
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