Western blot analysis of extracts of K562 cells, using PLA2G10 antibody (A10256) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PLA2G10 Rabbit pAb
Catalog No:AB-A10256
Manufacturer:ABclonal
Synonyms:PLA2G10;GXPLA2;GXSPLA2;SPLA2;sPLA2-X
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PLA2G10 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the phospholipase A2 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This calcium-dependent enzyme hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In one example, this enzyme catalyzes the release of arachidonic acid from cell membrane phospholipids, thus playing a role in the production of various inflammatory lipid mediators, such as prostaglandins. The encoded protein may promote the survival of breast cancer cells through its role in lipid metabolism. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PLA2G10 (NP_003552.1). |
Gene ID: | 8399 |
Swiss prot: | O15496 |
Molecular weight: | 18kDa |
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