Western blot analysis of extracts of various cell lines, using PKN1 antibody (A0553) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
PKN1 Rabbit pAb
Catalog No:AB-A0553
Manufacturer:ABclonal
Synonyms:PKN1;DBK;PAK-1;PAK1;PKN;PKN-ALPHA;PRK1;PRKCL1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PKN1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3). |
Gene ID: | 5585 |
Swiss prot: | Q16512 |
Molecular weight: | 104kDa |
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