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Western blot analysis of extracts of various cell lines, using PKA RIIα (PRKAR2A) Rabbit mAb (A3889) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
PKA RIIα (PRKAR2A) Rabbit mAb
Catalog No：AB-A3889
Manufacturer：ABclonal
Synonyms：PKR2, PRKAR2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | PKA RII伪 (PRKAR2A) Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human PKA RII伪 (PRKAR2A) |
| Gene ID: | 5576 |
| Swiss prot: | P13861 |
| Molecular weight: | 52kDa |
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