Western blot analysis of extracts of various cell lines, using PITX2 antibody (A1870) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
PITX2 Rabbit pAb
Catalog No:AB-A1870
Manufacturer:ABclonal
Synonyms:PITX2;ARP1;ASGD4;Brx1;IDG2;IGDS;IGDS2;IHG2;IRID2;Otlx2;PTX2;RGS;RIEG;RIEG1;RS
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PITX2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX2 (NP_000316.2). |
Gene ID: | 5308 |
Swiss prot: | Q99697 |
Molecular weight: | 30kDa/35kDa |
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